期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 260, 期 -, 页码 295-302出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.01.013
关键词
Fluorescein; Fluorescent probe; Glutathione; Cysteine; Cell imaging
资金
- National Natural Science Foundation of China [21504089, U1304202]
- Scientific and Technological Projects of Henan province [162300410209, 152102210343, 162300410236]
- Program for Science and Technology Innovation Talents in Universities of Henan Province [15HASTIT002]
- key project of education department of Henan Province [17A150050]
- Key Program in Universities of Henan Province [15B150016]
- Scientific and Technological Projects of Xuchang
- National Students' Platform for Innovation and Entrepreneurship Training Program [201710480001]
A new fluorescein-based probe (Py-F) was designed and synthesized to develop an optical and fluorescent sensor for biological thiols. Py-F possessed the thiol-responsed disulfide group and displayed selectivity and sensitivity for glutathione (GSH) and cysteine (Cys) over homocysteine (Hcy) in the CH3CN/PBS buffer solution, and the fluorescence detection limit was calculated to be 0.12 mu M and 0.13 mu M, respectively. Besides, the recognition mechanism was explained by density functional theory (DFT) calculation, and confirmed by mass spectroscope measurement. Furthermore, the thiols detection application in cellular imaging assays indicated that the probe has appreciable cell permeability and can serve as a fluorescent probe for detecting biothiols in living cells. (c) 2018 Elsevier B.V. All rights reserved.
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