期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 255, 期 -, 页码 2143-2150出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2017.09.018
关键词
Biosensor platform; Bloch surface waves; Label-free optical sensor; Enhanced fluorescence; Tumor biomarker; Complex matrices
资金
- European Commission through the project BILOBA [318035]
We report on the detection of an angiogenic molecule Vascular Endothelial Growth Factor (VEGF) in different biological matrices by means of a new integrated biosensing platform exploiting the properties of Bloch surface waves. The new platform takes advantage of a tandem configuration, in which both label-free and enhanced fluorescence detection are implemented. Specifically designed one dimensional photonic crystals were deposited directly on disposable and low cost plastic biochips. A direct sandwich immunoassay was used to detect VEGF in buffer, cell culture supernatant and human plasma at low concentration (ng/mL). The platform enabled the detection of VEGF in all three matrices with high resolution, fast turnaround time (30 min) and in close agreement with the results of enzyme linked immunosorbent assays. (C) 2017 Elsevier B.V. All rights reserved.
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