期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 259, 期 -, 页码 354-363出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2017.12.043
关键词
Dengue NS1; TEMPO; Quantitative point of care; Lateral flow; Electrochemistry; PEG modified gold nanoparticles
资金
- Singapore-MIT Alliance for Research and Technology (SMART) Innovation Grant [ING149091-BIO]
- National Research Foundation (NRF), Prime Minister's Office, Singapore
The development of a rapid, affordable, and sensitive diagnostic kit for point-of-care is important in most healthcare settings. In this follow-up paper to our previous work on quantification of dengue NS1 protein via impedimetric measurement, our present technology aims to provide quantification by utilizing proprietary stabilized and improved electroactive immunonanoparticles that bind to the target biomarker and subsequently move along toward the biofunctionalized screen-printed gold electrodes (SPGE) to generate an amperometric signal. The SPGE functions simultaneously as a signal transducer and a solid-state support for a sandwich ELISA-like immunoassay. The successful immunocomplex formation is then recorded electrochemically using a potentiostat, whereby the signal was contributed by the presence of a more hydrophilic redox label than ferrocene, namely radical TEMPO (TEMPO center dot), on the formulated nanoparticles. In this paper, a bifunctional ligand, thiolated polyethylene glycol (PEG-thiol) polymer, was used to stabilize 20 nm gold colloidal nanoparticles (AuNPs) in the formulation. PEG was incorporated to not only prevent the salt-mediated AuNPs aggregations, but also provide an anchor for antibody and redox species conjugation. To-date, we have successfully miniaturized a 3D-printed prototype device able to sensitively detect and quantify dengue NS1 protein with only 0.6 mu L human clinical serum samples diluted in a volume ratio of 1:100 (PBS diluent) in less than 30 min with a simple cyclic voltammetry analysis. The positive and negative samples were also tested with ELISA, a gold standard validation method, which means that the development of the prototype is a promising start as a point-of-care diagnostic technology. (C) 2017 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据