期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 257, 期 -, 页码 87-95出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2017.10.138
关键词
Electrochemiluminescence; Cytochrome c; Cerium oxide; Graphene; Apoptotic cell
资金
- Council of University of Tehran
Cytochrome c (Cyt c) is a small heme-containing metalloprotein which plays an important role in the electron transport chain in mitochondria. Monitoring Cyt c can be a key factor to find cell apoptosis process. In this work, a label-free electrochemiluminescence aptasensor for highly sensitive detection of Cyt c is introduced. We found that a new nano-based material composed of reduced graphene oxide/tris(2,2-bipyridyl) ruthenium(II) complex-cerium oxide nanoparticles-chitosan (RGO/Ru(bpy)(3)(2+)-CeO(2)NPs-CS) can be a remarkable sensing platform in designing an electrochemiluminescence sensor. Positively charged RGO/Ru(bpy)(3)(2+)-CeO(2)NPs-CS were placed on the surface of a Screen-Printed Carbon Electrode (SPCE), and then, negatively charged aptamer electrostatically immobilized on the modified electrode to form apta-CS-CeO(2)NPs-Ru(bpy)(3)(2+)-RGO/SPCE. Finally the surface of the electrode was incubated with BSA to block nonspecific binding sites. In the absence of Cyt c, a weak ECL signals was produced while in the presence of Cyt c, a remarkable increase in ECL signals was observed because of detaching the aptamer from the surface and formation of aptamer-Cyt c complex. The proposed aptasensor exhibits a wide dynamic range from 0.2-100 nM (R-2 = 0.993, n = 8) with a low detection limit of 25 pM which is applicable for the determination of Cyt c to find early stage apoptotic cells. (c) 2017 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据