期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 262, 期 -, 页码 452-459出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.02.044
关键词
Viscosity; Fluorescent probe; Mitochondria; Ratiometric; Two-photon
资金
- NSFC [21472067, 21502067, 21672083]
- Natural Science Foundation of Shandong Province, China [ZR2014BP001]
- Taishan Scholar Foundation [TS 201511041]
- University of Jinan [309-10004, 160082101]
Abnormal viscosity variations in the mitochondrial matrix may induce changes in the mitochondrial network organization and further influence metabolite diffusion, which, consequently, are related to cellular aspects of several diseases and malfunctions, such as cell malignancy, atherosclerosis, diabetes, and Alzheimer's disease. Studies have shown that mitochondrial viscosity changes are influenced by many factors, including disruption of ionic balance by drugs, over-production of reactive oxygen species, and cell apoptosis. However, the quantization of mitochondrial viscosity changes caused by these factors is uncertain, mainly because of the lack of a suitable fluorescent viscosity probe to accurately analyze changes in mitochondrial viscosity. By combining two fluorophores that have different sensitivities to viscosity, we designed and synthesized a new mitochondrial-targeted two-photon fluorescent viscosity probe (TM-V). The probe showed a direct linear relationship between the fluorescence intensity ratio of the two fluorophores and the media viscosity, which allowed us to determine the average mitochondrial viscosity in living cells. Using the novel TM-V probe, changes in mitochondrial viscosity under various conditions were quantified. In addition, as an excellent two-proton rotor probe, TM-V was able to report on viscosity in living cells and deeper tissues by ratiometric fluorescence imaging. (C) 2018 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据