期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 258, 期 -, 页码 813-821出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2017.11.161
关键词
Hollow carbon spheres; Polyaniline; Electrochemical biosensor; Acetylcholine esterase; Detection of malathion
资金
- Programs for the National Natural Science Foundation of China (NSFC) [U1604127, 21601161]
- Innovative Technology Team of Henan Province [CXTD2014042]
A novel electrochemical biosensor was constructed by anchoring acetylcholine esterase (AChE) onto the core-shell nanostructured composite of hollow carbon spheres coated with needle-like polyaniline(denoted by HCS@PANI), and used for rapid determination of malathion, which was established based on the chemisorption/desorption process of AChE used as an indicator. The uniformly distributed HCS was wrapped with the needle-like PANI nanowires to form the HCS@PANI nanocomposite, which not only exhibits high specific surface area and chemical functionality but also possesses strong electrochemical activity. After AChE was adsorbed onto the HCS@PANI nanocomposite, the electrochemical catalysis of AChE/HCS@PANI-based biosensor towards acetylthiocholine chloride (ATCl) was inhibited in the presence of malathion, which was applied to detect malathion, and the biosensor exhibited a low detection limit of 0.16 ng mL(-1) within a linear range from 1.0 ng mL(-1) to 10 mu g mL(-1) of the malathion concentration. Also, the results suggested that the developed electrochemical biosensor showed high selectivity, good reproducibility, and applicability for malathion detection, indicating that the proposed method using the HCS@PANI nanocomposite as the sensitive layer for the AChE immobilization and catalysis of ATCl has potential application in high-sensitive pesticide determinations. (c) 2017 Elsevier B.V. All rights reserved.
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