期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 266, 期 -, 页码 115-123出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.03.040
关键词
Surface enhanced Raman scattering; Immunoassay; Sensor; Mycotoxin; Multiplex; Foodstuff
资金
- Chinese Universities Scientific Fund [201510019065]
A surface enhanced Raman scattering (SERS) based immunosensor was developed for the detection of three mycotoxins (aflatoxin B-1, AFB(1); zearalenone, ZEA; ochratoxin A, OTA) in foodstuff. Gold nanoparticles (GNPs) were labeled with 5,5-dithiobis(succinimidyl-2-nitrobenzoate) (DSNB) as Raman reporter and covalently linked with anti-mycotoxin antibodies as SERS nanoprobes, while AFB(1)-BSA, ZEA-BSA, and OTA-BSA conjugates were covalently linked onto micro-array gold surface as corresponding capture addresses. This design allows three independent immunoreactions multiplexed on a single gold chip. After optimization, the limits of detection of the developed assay are 0.061-0.066 mu g/kg for AFB(1), 0.53-0.57 mu g/kg for ZEA, and 0.26-0.29 mu g/kg for OTA in foodstuff. The spiked experiments presented an acceptable assay accuracy with recovery of 83.8%-108.1% and a reasonable assay precision with variation of coefficient less than 15%. Furthermore, the analysis of actual samples by our method demonstrated consistent results by comparison with conventional instrumental analysis. These results indicate that the developed SERS immunosensor can be a promising tool for simultaneously and rapidly monitoring multiple-mycotoxin levels in foodstuff. (C) 2018 Elsevier B.V. All rights reserved.
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