On-chip plasmonic detection of microRNA-106a in gastric cancer using hybridized gold nanoparticles

For the early detection of miRNA-106a, the promising biomarker of gastric cancer, we demonstrate on-chip colorimetric biosensing platform based on the plasmon coupling of hybridized gold nanoparticles. Uniform and reproducible substrates are developed by optimizing the distribution and density of core gold nanoparticles with controlled ratio of organosilanes for optical on-chip detection. The core satellite nanostructure is made by a conjugation between miRNA-106a and probes functionalized on gold nanoparticles, thus enabling the observation of scattering color changes and spectral shift by plasmon coupling effect with high specificity and sensitivity. (C) 2018 Elsevier B.V. All rights reserved.