期刊
SENSORS
卷 18, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/s18072277
关键词
potentiometric sensor; surface potential; pH-sensitive sensor; full electrical readout; isothermal amplification; pathogenic bacteria; label-free
资金
- Ministry of Science, ICT & Future Planning (MISP) of Korea [H-GUARD_2013M3A6B2078]
- Korea Institute of Machinery and Materials [SC1290]
- National Research Council of Science & Technology (NST), Republic of Korea [SC1290] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2013M3A6B2078943] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
A disposable potentiometric sensor was newly developed for the amplification-coupled detection of nucleic acids. The hydrogen-ion is generally released during isothermal amplification of nucleic acids. The surface potential on the oxide-functionalized electrode of the extended gate was directly measured using full electrical circuits with the commercial metal-oxide semiconductor field-effect transistors (MOSFETs) and ring oscillator components, which resulted in cost-effective, portable and scalable real-time nucleic acid analysis. The current-starved ring oscillator changes surface potential to its frequency depending on the square of the variation in pH with a high signal-to-noise ratio during isothermal amplification. The device achieves a conversion rate of 20.5 kHz/mV and a detection resolution of 200 mu V for the surface potential. It is demonstrated that the sensor successfully monitors in real-time isothermal amplification of the extracted nucleic acids from Salmonella pathogenic bacteria. The in situ variations in the frequency of the pH-sensitive sensor were compared with the results of both a conventional optical device and pH-meter during isothermal amplification.
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