期刊
SENSORS
卷 18, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/s18040990
关键词
organic field-effect transistors; DNA hybridization detection; hairpin-shaped oligonucleotides; electronic sensors
资金
- Sardinia Regional Government (AMBROSIA) [CRP-59886]
- national flagship project NANOMAX
- project ENCODER
In this paper, the electronic transduction of DNA hybridization is presented by coupling organic charge-modulated field-effect transistors (OCMFETs) and hairpin-shaped probes. These probes have shown interesting properties in terms of sensitivity and selectivity in other kinds of assays, in the form of molecular beacons (MBs). Their integration with organic-transistor based sensors, never explored before, paves the way to a new class of low-cost, easy-to-use, and portable genetic sensors with enhanced performances. Thanks to the peculiar characteristics of the employed sensor, measurements can be performed at relatively high ionic strengths, thus optimizing the probes' functionality without affecting the detection ability of the device. A complete electrical characterization of the sensor is reported, including calibration with different target concentrations in the measurement environment and selectivity evaluation. In particular, DNA hybridization detection for target concentration as low as 100 pM is demonstrated.
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