期刊
SCIENTIA HORTICULTURAE
卷 238, 期 -, 页码 350-355出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.scienta.2018.05.003
关键词
Pear; Self-incompatibility; S-RNase; S-genotype; Pollen-part mutation
类别
资金
- National Natural Science Foundation of China [3167110219, 31772276]
Self-incompatibility is a genetic mechanism in flowering plants that promotes outcrossing and prevents inbreeding. In this study, the reported 15 S-RNase alleles were isolated from 18 pear accessions that contained 14 different S-genotypes. The S-RNase alleles were only expressed in the style, not in the root, stem, leaf, fruit, sepal, and pollen. Pollen tube grew into self-styles and self-pollinated fruit set involved the breakdown of self-incompatibility in cv. Xinxue. However, the two S-RNase alleles in cv. Xinxue had identical amino acid sequences to those self-incompatible cultivars, and S-5- and S-6-RNase were normally expressed in style, indicating that the loss of self-incompatibility in cv. Xinxue likely resulted from pollen-part mutation. Further, S5S5 and S6S6 genotyped individuals were identified in self-pollinated progeny, indicating that S-5 and S-6 genotyped pollen were compatible with the self-styles of cv. Xinxue. A genetic analysis showed that the segregation ratio of S5S5, S5S6, and S6S6 was approximately 0:2:1 (chi(2) = 3.505 chi(0.05, 2) =5.99), suggesting that the breakdown of self-incompatibility could be caused by modified factor(s) located outside of the S-locus. These results are useful for parental assignment and understanding self-incompatibility reaction.
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