期刊
SCIENCE OF THE TOTAL ENVIRONMENT
卷 645, 期 -, 页码 1230-1237出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.scitotenv.2018.07.260
关键词
Antibiotic resistance; Phyllosphere; Sludge; Chicken manure; Leaf-microbiome
资金
- National Key Research and Development Plan of China [2016YFD0800205, 2017YFE0107300]
- Natural Science Foundation of China [31722004]
- K.C. Wong Education Foundation
- Youth Innovation Promotion Association, CAS
Phyllosphere contains various microorganisms that may harbor diverse antibiotic resistance genes (ARGs). However, we know little about the composition of antibiotic resistome and the factors influencing the diversity and abundance of ARGs in the phyllosphere. In this study, 16S rRNA gene amplicon sequencing and high-throughput quantitative PCR approaches were employed to investigate the effects of long-term (over 10 years) organic fertilization on the phyllosphere bacterial communities and antibiotic resistome. Proteobacteria, Bacteroidetes, Actinobacteria and Firmicutes dominated in the phyllosphere bacterial communities. Long-term application of sewage sludge and chicken manure altered the phyllosphere bacterial community composition, with a remarkable decrease in bacterial alpha-diversity. A total of 124 unique ARGs were detected in the phyllosphere. The application of sewage sludge and chicken manure significantly increased the abundance of ARGs, with a maximum 2638-fold enrichment. Variation partitioning analysis (VPA) together with network analysis indicated that the profile of ARGs is strongly correlated with bacterial community compositions. These results improve the knowledge about the diversity of plant-associated antibiotic resistome and factors influencing the profile of ARGs in the phyllosphere. (C) 2018 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据