期刊
SCIENCE OF THE TOTAL ENVIRONMENT
卷 610, 期 -, 页码 602-612出版社
ELSEVIER
DOI: 10.1016/j.scitotenv.2017.05.190
关键词
Experimental design; Abiotic factors; GC-MS; Metabolomics; Daphnia magna; Chemometrics
资金
- European Research Council under the European Union [320737]
- European Research Council (ERC) [320737] Funding Source: European Research Council (ERC)
Metabolic changes of Daphnia magna pools due different abiotic factors linked to global climate change (salinity, temperature and hypoxia) were investigated using untargeted GC-MS and advanced chemometric strategies using a three factors two-level full factorial experimental design (DoE). Effects of these three factors and identity of the metabolites whose concentrations changed because of them were investigated. The simultaneous analysis of GC-MS data sets using Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) allowed the resolution of the elution and mass spectra profiles of a large number of D. magna metabolites. Changes in peak areas of these metabolites were then analyzed by Principal Component Analysis (PCA), by ANOVA-Simultaneous Component Analysis (ASCA) and by Orthogonal Partial Least Squares-Discriminant Analysis (OPLS-DA), and the combined effects of the three investigated stressors were assessed. Results confirmed the strong influence of increasing environmental salinity levels on the D. magna metabolome. This impact was specially highlighted by changes on the cellular content of carbohydrates, fatty acids, organic acids and amino acid molecules. In contrast, these effects were less significant for the other two factors (temperature and hypoxia) at the moderate stressing experimental conditions investigated in this work when they were not combined with salinity. (C) 2017 Elsevier B.V. All rights reserved.
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