期刊
SCIENCE
卷 359, 期 6377, 页码 779-782出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aao0157
关键词
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资金
- Swiss National Science Foundation [31003A_146992/1]
- European Union (SYNPEPTIDE)
- European Union (European Research Council Advanced Grant SynPlex)
- Helmut Horten Foundation
- Alexander von Humboldt Fellowship
- ETH Postdoctoral Fellowship
- Institut Pasteur
Current textbook knowledge holds that the structural scope of ribosomal biosynthesis is based exclusively on alpha-amino acid backbone topology. Here we report the genome-guided discovery of bacterial pathways that posttranslationally create beta-amino acid-containing products. The transformation is widespread in bacteria and is catalyzed by an enzyme belonging to a previously uncharacterized radical S-adenosylmethionine family. We show that the beta-amino acids result from an unusual protein splicing process involving backbone carbon-carbon bond cleavage and net excision of tyramine. The reaction can be used to incorporate diverse and multiple beta-amino acids into genetically encoded precursors in Escherichia coli. In addition to enlarging the set of basic amino acid components, the excision generates keto functions that are useful as orthogonal reaction sites for chemical diversification.
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