期刊
SCIENCE
卷 360, 期 6388, 页码 506-+出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aap7847
关键词
-
资金
- Wellcome Trust grant [098051]
- National Institutes of Health grants [R01 AI094973, R01 AI117017, F32 AI112271]
Severe malaria is caused by the apicomplexan parasite Plasmodium falciparum. Despite decades of research, the distinct biology of these parasites has made it challenging to establish high-throughput genetic approaches to identify and prioritize therapeutic targets. Using transposon mutagenesis of P. falciparum in an approach that exploited its AT-rich genome, we generated more than 38,000 mutants, saturating the genome and defining mutability and fitness costs for over 87% of genes. Of 5399 genes, our study defined 2680 genes as essential for optimal growth of asexual blood stages in vitro. These essential genes are associated with drug resistance, represent leading vaccine candidates, and include approximately 1000 Plasmodium-conserved genes of unknown function. We validated this approach by testing proteasome pathways for individualmutants associated with artemisinin sensitivity.
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