期刊
CRITICAL CARE
卷 16, 期 5, 页码 -出版社
BIOMED CENTRAL LTD
DOI: 10.1186/cc11838
关键词
-
资金
- Programme Hospitalier de Recherche Clinique (PHRC)
- Biomerieux-Institut Pasteur collaborative research program
- French public agency, OSEO
- Institut Pasteur
- Paris University
- Pasteur-Weizmann fellowship
Introduction: During the course of infection, natural killer (NK) cells contribute to innate immunity by producing cytokines, particularly interferon-gamma (IFN-gamma). In addition to their beneficial effects against infection, NK cells may play a detrimental role during systemic inflammation, causing lethality during sepsis. Little is known on the immune status of NK cells in patients with systemic inflammatory response syndrome (SIRS) or sepsis in terms of cell surface markers expression and IFN-gamma production. Methods: We investigated 27 sepsis patients and 11 patients with non-infectious SIRS. CD56(bright) and CD56(dim) NK cell subsets were identified by flow cytometry and Toll-like receptor (TLR) 2, TLR4, TLR9, CX3CR1, CD16 and CD69 expression were analyzed, as well as ex vivo IFN-gamma production by NK cells in whole blood samples. Results: We first showed that in NK cells from healthy controls, TLR2 and TLR4 expression is mainly intracellular, similarly to TLR9. Intracellular levels of TLR2 and TLR4, in both CD56(bright) and CD56(dim) NK cell subsets from sepsis patients, were increased compared to healthy subjects. In addition, the percentage of CD69(+) cells was higher among NK cells of sepsis patients. No difference was observed for TLR9, CX3CR1, and CD16 expression. The ex vivo stimulation by TLR4 or TLR9 agonists, or whole bacteria in synergy with accessory cytokines (IL-15+IL-18), resulted in significant production of IFN-gamma by NK cells of healthy controls. In contrast, for SIRS and sepsis patients this response was dramatically reduced. Conclusions: This study reports for the first time an intracellular expression of TLR2 and TLR4 in human NK cells. Surface TLR4 expression allows discriminating sepsis and SIRS. Furthermore, during these pathologies, NK cells undergo an alteration of their immune status characterized by a profound reduction of their capacity to release IFN-gamma.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据