期刊
RNA BIOLOGY
卷 15, 期 8, 页码 1032-1039出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15476286.2018.1435248
关键词
Circular RNA; Hepatitis C Virus; microRNA-122; molecular sponge; RNA therapy
资金
- LOEWE program Medical RNomics (State of Hessen)
- DFG [SFB 1021]
Circular RNAs (circRNAs) were recently described as a novel class of cellular RNAs. Two circRNAs were reported to function as molecular sponges, sequestering specific microRNAs, thereby de-repressing target mRNAs. Due to their elevated stability in comparison to linear RNA, circRNAs may be an interesting tool in molecular medicine and biology. In this study, we provide a proof-of-principle that circRNAs can be engineered as microRNA sponges. As a model system, we used the Hepatitis C Virus (HCV), which requires cellular microRNA-122 for its life cycle. We produced artificial circRNA sponges in vitro that efficiently sequester microRNA-122, thereby inhibiting viral protein production in an HCV cell culture system. These circRNAs are more stable than their linear counterparts, and localize both to the cytoplasm and to the nucleus, opening up a wide range of potential applications.
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