期刊
REPRODUCTIVE TOXICOLOGY
卷 75, 期 -, 页码 96-109出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.reprotox.2017.12.005
关键词
alpha-Solanine; Oocyte maturation; Autophagy; Apoptosis; Histone modifications
资金
- BioGreen 21 Program [PJ01119601]
- Cooperative Research Program for Agriculture Science & Technology Development of the Rural Development Administration [PJ0118512016]
- Bio-industry Technology Development Program of the Ministry of Agriculture, Food and Rural Affairs, Republic of Korea [IPET312060-5]
In this study, we used a pig model to investigate the effects of alpha-solanine (a natural toxin found mainly in potato sprouts) on oocyte maturation, quality and subsequent embryonic development. We found that alpha-solanine (10 mu M) disturbed meiotic resumption and increased abnormal spindle formation and altered the cortical granule (CG) distribution compared with the untreated group. alpha-Solanine triggered autophagy and apoptosis by increasing the expressions of autophagy-related genes (LC3, ATG7, and LAMP2) and apoptotic related genes (BAX and CASP3). Exposure of porcine oocytes to alpha-solanine significantly increased the levels of H3K36me3 and H3K27me3. Moreover, alpha-solanine significantly reduced the cleavage and blastocyst formation rates, decreased the total and inner cell mass cells numbers, and increased apoptosis in these porcine embryos. Taken together, our data indicate that alpha-solanine toxically impairs oocyte maturation and quality by triggering autophagy/apoptosis and facilitating epigenetic modifications. Furthermore, alpha-solanine suppressed subsequent embryonic development and reduced embryo quality. (C) 2017 Elsevier Inc. All rights reserved.
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