期刊
RENEWABLE ENERGY
卷 118, 期 -, 页码 14-24出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.renene.2017.11.001
关键词
Corn stover; Rice straw; On-site cellulase production; Recombinant Saccharomyces cerevisiae; Adapted Pichia stipistis; Bioethanol
资金
- Youth Fund from Jiangsu Province [BK20150130]
- China Postdoctoral Science Foundation [2016M600815, 2017M613213]
- Fund for Doctoral Scientific Research [Z109021632]
- Start-up Fund for Talent Introduction from Northwest AF University [Z111021602]
- Natural Science Foundation of China (NSFC) [31400246]
- Natural Science Foundation of Shaanxi [2015JQ3087]
- Northwest A&F University Basic Research Foundation [2014YB039]
A novel and efficient bioprocess from sodium hydroxide pretreated corn stover (SHPCS) or sodium hydroxide pretreated rice straw (SHPRS) to ethanol was successfully established, where the on-site cellulase production by the mixed culture of Trichoderma reesei and Aspergillus niger was used, producing 3.63 +/- 035 FPIU/mL cellulase from SHPCS and 2.56 +/- 0.33 FPIU/mL cellulase from SHPRS, respectively. Then those cellulases were applied to the enzymatic saccharification of SHPCS and SHPRS respectively, leading to yields of 81.5 +/- 1.2% and 70.5 +/- 2.1% respectively. Subsequently, SHPCS and SHPRS enzymatic hydrolysates mainly containing glucose and xylose were fermented by Saccharomyces cerevisiae, producing 27.6 +/- 1.5 and 21.7 +/- 1.9 g/L ethanol respectively. Concurrent with the distillation of fermentation broths for ethanol separation, the residual xylose was concentrated about 3 times. Thereafter, the distillation residues from SHPCS and SHPRS containing xylose Were fermented by the adapted Pichia stipistis, producing 16.6 +/- 1.1 and 13.0 +/- 0.9 g/L ethanol respectively. In sum, the bioprocess could produce 33.1 g ethanol from 113.20 g SHPCS and 26.0 g ethanol from 117.58 g SHPRS. (C) 2017 tIsevier Ltd. All rights reserved.
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