期刊
RENAL FAILURE
卷 40, 期 1, 页码 273-279出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/0886022X.2018.1456460
关键词
Podocyte; puromycin aminonucleoside; Salvia przewalskii extract of total phenolic acids; salvianolic acid B; rosmarinic acid; TLR4
资金
- Technical Innovation Foundation Projects from Nanjing Military Region Medical Scientific [15DX008]
Background: TLR4 signaling is known to be involved in podocyte injury. We have previously shown that Salvia przewalskii extract of total phenolic acids (SPE) and its active monomer salvianolic acid B (SalB) and rosmarinic acid (RA) protect podocytes from injury induced by PAN. In the present study, we test whether SPE inhibits TLR4 signaling.Methods: The conditionally immortalized mouse podocytes were treated with SPE, SalB, RA, SalB+RA or tacrolimus for 30min, followed by PAN (100g/mL) for 24h. The F-actin staining with phalloidin was used to assess cytoskeletal injury in the podocytes. Western blotting and semi-quantitatives RT-PCR were used to assess the changes of the components in the TLR4 signaling pathway.Results: (1) The F-actin stress fibers of podocytes were almost completely disrupted after PAN treatment for 24h, and the disruption was significantly alleviated by SPE; (2) the PAN-induced elevation of mRNA levels of TLR4, MyD88 and p65 were inhibited except p65 with high-dose SalB; (3) consistently, the protein levels of TLR4, MyD88 and pp65 were significantly elevated by PAN, and SPE, SalB, RA and admixture, respectively, attenuated the elevations of TLR4 and pp65 proteins; (4) SPE and tacrolimus have a similarly strong effect on inhibition of the expression of TLR4 signaling components.Conclusions: SPE protects podocytes from PAN-induced injury at least partly through inhibiting TLR4 signaling. SPE is as strong as tacrolimus in inhibiting TLR4 signaling in podocytes.
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