期刊
QUIMICA NOVA
卷 41, 期 3, 页码 297-301出版社
SOC BRASILEIRA QUIMICA
DOI: 10.21577/0100-4042.20170184
关键词
Phellodendri Cortex; isoquinoline alkaloids; palmatine; berberine; pH-Zone-Refining Counter-Current Chromatography
资金
- Science Foundation of China [21506119]
- Shandong Province Major Scientific and Technological Innovation Project [2017CXGC1308]
- Primary Research & Developement Plan of Shandong Province [2017GSF216002]
- Three-academy Joint Funds of Shandong Provincial Natural Science Foundation [ZR2016YL006]
pH-Zone-refining counter-current chromatography (PZRCCC) was successfully used for preparative separation isoquinoline alkaloids from Phellodendri Cortex. Two target compounds of berberine and palmatine were completely resolved by a two-phase solvent system chloroform/methanol/water (4:2:2, v/v), with 10 mmol L-1HCl in upper stationary phase and 10 mmol L-1 TEA in lower organic phase. From 2.0 g of the crude alkaloids, palmatine (448 mg,95.1%) and berberine (159 mg, 95.4%) were obtained in one-step PZRCCC separation. The chemical structures were identified by ESI-MS and NMR data. The results indicated that PZRCCC separation is an excellent preparative separation method for isoquinoline alkaloids from Phellodendri Cortex.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据