期刊
PULMONARY PHARMACOLOGY & THERAPEUTICS
卷 49, 期 -, 页码 54-59出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.pupt.2018.01.005
关键词
Obesity; Estrogen; Lung injury; Intestinal ischemia and reperfusion; Inflammation; Female mice
资金
- Grant Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/King's College London [FAPESP/KCL 2010/51330-2, FAPESP/KCL 2012/50550-4]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [10/51330-2, 12/50550-4] Funding Source: FAPESP
Rational: Acute lung injury (ALI) is a common complication after intestinal ischemia and reperfusion (I/R) injury that can lead to acute respiratory distress syndrome (ARDS). We have previously demonstrated that females are protected against lung damage induced by intestinal I/R through an estrogen mediated mechanism. Objectives: To investigate the effect of obesity on ALI induced by intestinal I/R in female mice. Methods: C57B1/6 female mice were fed with a standard low-fat diet (SD) or a high-fat diet (HFD) for 9 weeks. Intestinal I/R injury was induced by a 45 min occlusion of the mesenteric artery followed by 2 and 24 h of reperfusion. Results: Significant increase in lung myeloperoxidase expression (MPO) and neutrophil numbers of SD and HFD mice occurred at 2 h and 24 h of reperfusion. Furthermore, HFD mice presented a significant increase in lung eosinophil peroxidase (EPO) expression and eosinophil numbers compared to SD mice. Lung wet/dry weight ratio was significantly greater in HFD mice at 2 and 24 h of reperfusion, accompanied by a significant increase in the expression of inducible NO in the lung tissue and a significant decrease in arterial oxygen saturation at 24 h of reperfusion relative to SD mice. Conclusion: Obesity predisposes female mice to increased pulmonary oedema and deterioration in gas exchange, which is accompanied by an increase in iNOS expression in the lung.
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