期刊
PROTEOMICS CLINICAL APPLICATIONS
卷 12, 期 3, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/prca.201700085
关键词
autism; biomarkers; iTRAQ; plasma; proteomics
资金
- NSFC (National Natural Science Foundation of China) [81501213]
- Shenzhen Bureau of Science, Technology and Information [JCYJ20140418095735542, JCYJ20150402100258220, GRCK2016041315403277, JCYJ20150529164656093, JCYJ20170412110026229]
- Shenzhen University students' innovative development fund [S2015017]
PurposeAutism is a childhood neurological disorder with poorly understood etiology and pathology. This study is designed to identify differentially expressed proteins that might serve as potential biomarkers for autism. Experimental designWe perform iTRAQ (isobaric tags for relative and absolute quantitation) analysis for normal and autistic children's plasma of the same age group. ResultsThe results show that 24 differentially expressed proteins were identified between autistic subjects and controls. For the first time, differential expression of complement C5 (C5) and fermitin family homolog 3 (FERMT3) are related to autism. Five proteins, that is, complement C3 (C3), C5, integrin alpha-IIb (ITGA2B), talin-1 (TLN1), and vitamin D-binding protein (GC) were validated via enzyme-linked immunosorbent assay (ELISA). By ROC (receiver operating characteristic) analysis, combinations of these five proteins C3, C5, GC, ITGA2B, and TLN1 distinguished autistic children from healthy controls with a high AUC (area under the ROC curve) value (0.982, 95% CI, 0.957-1.000, p<0.000). ConclusionThese above described proteins are found involved in different pathways that have previously been linked to the pathophysiology of autism spectrum disorders (ASDs). The results strongly support that focal adhesions, acting cytoskeleton, cell adhesion, motility and migration, synaptogenesis, and complement system are involved in the pathogenesis of autism, and highlight the important role of platelet function in the pathophysiology of autism.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据