期刊
PROTEOMICS
卷 18, 期 2, 页码 -出版社
WILEY
DOI: 10.1002/pmic.201700277
关键词
antibody isotype; malaria; multiplex; protein microarray; quantum dots
资金
- National Institute of Allergy and Infectious Diseases
- National Institutes of Health, Department of Health and Human Services [HHSN272201200031C]
- R01 grant [AI-095916]
- U19 grant [AI089686]
- National Center for Research Resources [ORIP/OD P51OD011132]
The development of vaccines against malaria and serodiagnostic tests for detecting recent exposure requires tools for antigen discovery and suitable animal models. The protein microarray is a high-throughput, sample sparing technique, with applications in infectious disease research, clinical diagnostics, epidemiology, and vaccine development. We recently demonstrated Qdot-based indirect immunofluorescence together with portable optical imager ArrayCAM using single isotype detection could replicate data using the conventional laser confocal scanner system. We developed a multiplexing protocol for simultaneous detection of IgG, IgA, and IgM and compared samples from a controlled human malaria infection model with those from controlled malaria infections of Aotus nancymaae, a widely used non-human primate model of human malaria. IgG profiles showed the highest concordance in number of reactive antigens; thus, of the 139 antigens recognized by human IgG antibody, 111 were also recognized by Aotus monkeys. Interestingly, IgA profiles were largely non-overlapping. Finally, on the path toward wider deployment of the portable platform, we show excellent correlations between array data obtained in five independent laboratories around the United States using the multiplexing protocol (R-2: 0.60-0.92). This study supports the use of this platform for wider deployment, particularly in endemic areas where such a tool will have the greatest impact on global human health.
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