期刊
PROTEIN SCIENCE
卷 27, 期 8, 页码 1427-1438出版社
WILEY
DOI: 10.1002/pro.3434
关键词
Amyloid beta-protein (A); Alzheimer disease; Raman spectroscopy; protein aggregation; protein assembly; protein misfolding; biosensor
资金
- Alexander von Humboldt-Stiftung
Amyloid beta-protein (A) self-association is one process linked to the development of Alzheimer's disease (AD). A peptides, including its most abundant forms, A40 and A42, are associated with the two predominant neuropathologic findings in AD, vascular and parenchymal amyloidosis, respectively. Efforts to develop therapies for AD often have focused on understanding and controlling the assembly of these two peptides. An obligate step in these efforts is the monitoring of assembly state. We show here that surface-enhanced Raman spectroscopy (SERS) coupled with principal component analysis (PCA) readily distinguishes A40 and A42. We show further, through comparison of assembly dependent changes in secondary structure and morphology, that the SERS/PCA approach unambiguously differentiates closely related assembly stages not readily differentiable by circular dichroism spectroscopy, electron microscopy, or other techniques. The high discriminating power of SERS/PCA is based on the rich structural information present in its spectra, which comprises not only on interatomic resonances between covalently associated atoms and hydrogen bond interactions important in controlling secondary structure, but effects of protein orientation relative to the substrate surface. Coupled with the label-free, single molecule sensitivity of SERS, the approach should prove useful for determining structure activity relationships, suggesting target sites for drug development, and for testing the effects of such drugs on the assembly process. The approach also could be of value in other systems in which assembly dependent changes in protein structure correlate with the formation of toxic peptide assemblies.
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