期刊
PROTEIN EXPRESSION AND PURIFICATION
卷 144, 期 -, 页码 25-32出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pep.2017.11.002
关键词
Adventitious viruses; Baculovirus insect cell system; Insect cell lines; Sf-rhabdovirus; Spodoptera frugiperda cells
类别
资金
- National Institutes of Health, Institutes of General Medical Sciences and Allergy and Infectious Diseases [R43 GM102982, R43 A1112118]
Insect cells are widely used for recombinant protein expression, typically as hosts for recombinant baculovirus vectors, but also for plasmid-mediated transient transfection or stable genetic transformation. Insect cells are used to express proteins for research, as well as to manufacture biologicals for human and veterinary medicine. Recently, several insect cell lines used for recombinant protein expression were found to be persistently infected with adventitious viruses. This has raised questions about how these infections might affect research performed using those cell lines. Furthermore, these findings raised serious concerns about the safety of biologicals produced using those cell lines. In response, new insect cell lines lacking adventitious viruses have been isolated for use as improved research tools and safer biological manufacturing platforms. Here, we review the scientific and patent literature on adventitious viruses found in insect cell lines, affected cell lines, and new virus-free cell lines.
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