4.6 Article

Influence of enzymatic hydrolysis conditions on the degree of hydrolysis and functional properties of protein hydrolysate obtained from Chinese sturgeon (Acipenser sinensis) by using papain enzyme

期刊

PROCESS BIOCHEMISTRY
卷 67, 期 -, 页码 19-28

出版社

ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2018.01.009

关键词

Sturgeon; Fish protein hydrolysate; Degree of hydrolysis; Functional properties; Papain

资金

  1. Natural Science Foundation of Jiangsu Province [BK20150152]
  2. earmarked fund for China Agriculture Research System [CARS-45-26]
  3. program of Collaborative innovation center of food safety and quality control in Jiangsu Province

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In this study, protein hydrolysate was prepared from the muscles of Chinese sturgeon (Acipenser sinensis). The effects of different conditions on the degree of hydrolysis (DH) by using papain were investigated. The DH of 24.89% was attained under the optimum conditions including solid-to-liquid mixing ratio of 1:1, enzyme substrate ratio of 3%, pH 6, temperature of 70 degrees C, and incubation time of 6 h. The yield of protein hydrolysate was 17.47%, in which the protein content was 79.67% and amino acid content was 96.35%. The molecular weight of peptides decreased with the progress in hydrolysis time. Protein hydrolysate solubility ranged between 86.57% and 98.74%, emulsifying activity index was 11.0-13.27 m(2)/g, emulsion stability index was > 94% at different pH levels, water holding capacity was 1.93 g water/g protein, oil holding capacity was 2.59 g oil/g protein, and foam capacity was 76.67%. The obtained fish protein hydrolysate contains improved functional properties and has potential applications in food industries.

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