期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 115, 期 26, 页码 6697-6702出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1806351115
关键词
Archaea; primase; DNA replication; DNA polymerase; Sulfolobus
资金
- College of Arts and Sciences, Indiana University
- NIH [1R01GM125579-01]
- Wellcome Trust Investigator Award [104641/Z/14/Z]
- Boehringer-Ingelheim Fonds
- Janggen-Pohn-Stiftung
- Swiss National Science Foundation
- Wellcome Trust [104641/Z/14/Z] Funding Source: Wellcome Trust
The cellular replicative DNA polymerases cannot initiate DNA synthesis without a priming 3' OH. During DNA replication, this is supplied in the context of a short RNA primer molecule synthesized by DNA primase. The primase of archaea and eukaryotes, despite having varying subunit compositions, share sequence and structural homology. Intriguingly, archaeal primase has been demonstrated to possess the ability to synthesize DNA de novo, a property shared with the eukaryotic PrimPol enzymes. The dual RNA and DNA synthetic capabilities of the archaeal DNA primase have led to the proposal that there may be a sequential hand-off between these synthetic modes of primase. In the current work, we dissect the functional interplay between DNA and RNA synthetic modes of primase. In addition, we determine the key determinants that govern primer length definition by the archaeal primase. Our results indicate a primer measuring system that functions akin to a caliper.
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