期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 115, 期 4, 页码 668-673出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1716514115
关键词
bacteria inactivation; fluorescence spectroscopy; PCA
资金
- Welch Foundation [1501928]
- Texas A&M Engineering Experiment Station funds
- National Natural Science Foundation of China [61222509, 11421064]
- Interdisciplinary Program of Shanghai Jiao Tong University [YG2015MS10]
The determination of live and dead bacteria is of considerable significance for preventing health care-associated infection in hospitals, field clinics, and other areas. In this study, the viable (live) and nonviable (dead) bacteria in a sample were determined by means of their fluorescence spectra and principal component analysis (PCA). Data obtained in this study show that it is possible to identify bacteria strains and determine the live/dead ratio after UV light inactivation and antibiotic treatment, in situ, within minutes. In addition, synchronous fluorescence scans enable the identification of bacterial components such as tryptophan, tyrosine, and DNA. Compared with the time-consuming plating and culturing methods, this study renders a means for rapid detection and determination of live and dead bacteria.
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