期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 115, 期 19, 页码 E4368-E4376出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1717920115
关键词
RNA Polymerase II; transcription; transcription dynamics; promoter-proximal pausing; live-cell imaging
资金
- Dutch Cancer Society [10506/2016-1]
- Dutch Organization for Scientific Research ZonMW TOP Grant [912.12.132]
- TOP ALW Grant [854.11.002]
- Horizon Zenith Grant [935.11.042]
- VIDI ALW Grant [864.13.004]
- Erasmus Medical Center fellowship
- Dutch Cancer Society
Initiation and promoter-proximal pausing are key regulatory steps of RNA Polymerase II (Pol II) transcription. To study the in vivo dynamics of endogenous Pol II during these steps, we generated fully functional GFP-RPB1 knockin cells. GFP-RPB1 photobleaching combined with computational modeling revealed four kinetically distinct Pol II fractions and showed that on average 7% of Pol II are freely diffusing, while 10% are chromatin-bound for 2.4 seconds during initiation, and 23% are promoter-paused for only 42 seconds. This unexpectedly high turnover of Pol II at promoters is most likely caused by premature termination of initiating and promoter-paused Pol II and is in sharp contrast to the 23 minutes that elongating Pol II resides on chromatin. Our live-cell-imaging approach provides insights into Pol II dynamics and suggests that the continuous release and reinitiation of promoter-bound Pol II is an important component of transcriptional regulation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据