期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 115, 期 30, 页码 7789-7794出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1722295115
关键词
circadian misalignment; shift work; diabetes; insulin sensitivity; skeletal muscle
资金
- Netherlands Organization for Scientific Research Grant TOP [40-00812-98-14047]
- Netherlands Cardiovascular Research Initiative
- Dutch Heart Foundation [CVON2014-02]
- School of Nutrition and Translational Research in Metabolism (NUTRIM) Netherlands Organization for Scientific Research (NWO) Graduate Programme
- Netherlands Organization for Scientific Research Grant [022.003.011]
- Vidi Grant from NWO [917.14.358]
- Senior Fellowship from Dutch Diabetes Research Foundation [2013.82.1639]
- NIH [R01HL118601, R01DK099512, R01DK105072, R01DK102696, R01HL140574]
- FP7 grant Eurhythdia and holds ERC advanced Grant [694717]
Circadian misalignment, such as in shift work, has been associated with obesity and type 2 diabetes. However, direct effects of circadian misalignment on skeletal muscle insulin sensitivity and the muscle molecular circadian clock have never been studied in humans. Here, we investigated insulin sensitivity and muscle metabolism in 14 healthy young lean men [age 22.4 +/- 2.8 years; body mass index (BMI) 22.3 +/- 2.1 kg/m(2) (mean +/- SD)] after a 3-d control protocol and a 3.5-d misalignment protocol induced by a 12-h rapid shift of the behavioral cycle. We show that short-term circadian misalignment results in a significant decrease in muscle insulin sensitivity due to a reduced skeletal muscle nonoxidative glucose disposal (rate of disappearance: 23.7 +/- 2.4 vs. 18.4 +/- 1.4 mg/kg per minute; control vs. misalignment; P = 0.024). Fasting glucose and free fatty acid levels as well as sleeping metabolic rate were higher during circadian misalignment. Molecular analysis of skeletal muscle biopsies revealed that the molecular circadian clock was not aligned to the inverted behavioral cycle, and transcriptome analysis revealed the human PPAR pathway as a key player in the disturbed energy metabolism upon circadian misalignment. Our findings may provide a mechanism underlying the increased risk of type 2 diabetes among shift workers.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据