期刊
METABOLITES
卷 3, 期 1, 页码 47-71出版社
MDPI
DOI: 10.3390/metabo3010047
关键词
metabolism; human milk; gas-chromatography mass-spectrometry; cyclodextrin; enantiomers; chiral chromatography
1,8-Cineole is a widely distributed odorant that also shows physiological effects, but whose human metabolism has hitherto not been extensively investigated. The aim of the present study was, thus, to characterise the metabolites of 1,8-cineole, identified previously in human milk, after the oral intake of 100 mg of this substance. Special emphasis was placed on the enantiomeric composition of the metabolites since these data may provide important insights into potential biotransformation pathways, as well as potential biological activities of these substances, for example on the breastfed child. The volatile fraction of the human milk samples was therefore isolated via Solvent Assisted Flavour Evaporation (SAFE) and subjected to gas chromatography-mass spectrometry (GC-MS). The absolute concentrations of each metabolite were determined by matrix calibration with an internal standard, and the ratios of enantiomers were analysed on chiral capillaries. The concentrations varied over a broad range, from traces in the upper ng/kg region up to 40 mu g/kg milk, with the exception of the main metabolite alpha 2-hydroxy-1,8-cineole that showed concentrations of 100-250 mu g/kg. Also, large inter- and intra-individual variations were recorded for the enantiomers, with nearly enantiomerically pure alpha 2-hydroxy- and 3-oxo-1,8-cineole, while all other metabolites showed ratios of similar to 30:70 to 80:20.
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