期刊
POLYMER DEGRADATION AND STABILITY
卷 152, 期 -, 页码 75-85出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.polymdegradstab.2018.03.023
关键词
Polylactic acid; Biodegradation; Biofilm formation; Stenotrophomonas pavanii; Pseudomonas geniculata
资金
- Thailand Research Fund (TRF) under a Directed Basic Research Grant [DBG6080004]
Enumeration and isolation of polylactic acid (PLA)-degrading bacteria from soils and wastewater sludge were performed on emulsified PLA agar. Two isolates of potent PLA-degrading bacteria, designated as CH1 and WS3, were selected and identified as Stenotrophomonas pavanii and Pseudomonas geniculata, respectively. PLA was presented as a substrate to stimulate production of protease and PLA-degrading enzyme by S. pavanii CHI and P. geniculata WS3. The optimal pH values for both protease and PLAdegrading enzyme production by S. pavanii CHI and P. geniculata WS3 were 7.5 and 8.0, respectively. The optimal gelatin concentrations for stimulating protease production in S. pavanii CHI and P. geniculata WS3 were 0.3% (w/v), while those for PLA-degrading enzyme production in S. pavanii CHI and P. geniculata WS3 were 0.1 and 0.3% (w/v), respectively. In addition, P. geniculata WS3 had a higher percentage of PLA film-weight loss than that of S. pavanii CHI, corresponding with reduced molecular weight of PLA. A significant increase of lactic acid content in culture broth was directly correlated with the increasing percentage of PLA film-weight loss. Our results clearly demonstrated that P. geniculata WS3, a novel isolated bacterium, has played a substantial role in PLA biodegradation by producing PLAdegrading enzyme and adhering on PIA surface. (C) 2018 Elsevier Ltd. All rights reserved.
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