Bioresorbable antibacterial PCL-PLA-nHA composite membranes for oral and maxillofacial defects

Degradable implant membranes designed to separate hard and soft tissues and to trigger the growth of underlying bone and with antimicrobial properties are needed for the management of bone defects, ridge augmentation and to facilitate dental implants. In this study, the composite scaffolds of poly(lactic acid) (PLA), poly(caprolactone) (PCL) blended with nano hydroxyapatite and cefixime-beta cyclodextrin (Cfx-beta CD) inclusion complexes were synthesized by electrospinning. The prepared electrospun fibrous membranes were characterized by scanning electron microscopy and Fourier transform infrared (FTIR) spectroscopy. Membranes were microporous with random fibers in the range of 0.2-0.37 mu m. The data from FTIR spectral analysis helped to characterize the presence of PCL, PLA, Cfx, and beta CD in the electrospun membranes. In addition, the mechanical properties (i.e., elastic modulus and tensile strength) of the scaffolds were investigated. The mechanical strength and suture retention ability of the membranes was comparable to that of skin grafts. Drug release assays confirmed the slow release of Cfx from the membranes in the presence of beta CD and antimicrobial studies showed that the membranes possessed antibacterial properties. The interaction of cells with membranes was evaluated by culturing them with the mouse pre-osteoblast cell line MC3T3 and assessment of bone formation was done using Alizarin Red Assay. Culturing MC3T3 cells on the scaffolds showed that cells attached and entered the membranes and increased in number over time. In summary, these membranes are flexible, strong, bactericidal and osteogenic, which are the ideal implant properties for dental and maxillofacial surgery. POLYM. COMPOS., 40:1564-1575, 2019. (c) 2018 Society of Plastics Engineers