期刊
PLOS ONE
卷 13, 期 4, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0196255
关键词
-
资金
- National Modern Agricultural Industry Special Program - Ministry of Agriculture of the Peoples Republic of China [CARS-38]
- Ministry of Finance of the Peoples Republic of China
- National 863 Program of China - Ministry of Science and Technology of the Peoples Republic of China [2013AA102505]
- National Science and Technology Support Projects - Ministry of Science and Technology of the Peoples Republic of China [2015BAD03B04]
- Shaanxi Technological Innovation Engineering Program - Department of Science and Technology of Shaanxi Province [2014KTZB02-02-01]
- National Natural Science Foundation of China - National Natural Science Foundation of China [31501937]
Myocyte enhancer factor 2A (MEF2A) is widely distributed in various tissues or organs and plays crucial roles in multiple biological processes. To examine the potential effects of MEF2A on skeletal muscle myoblast, the functional role of MFE2A in myoblast proliferation and differentiation was investigated. In this study, we found that the mRNA expression level of Mef2a was dramatically increased during the myogenesis of bovine skeletal muscle primary myoblast. Overexpression of MEF2A significantly promoted myoblast proliferation, while knockdown of MEF2A inhibited the proliferation and differentiation of myoblast. RT-PCR and western blot analysis revealed that this positive effect of MEF2A on the proliferation of myoblast was carried out by triggering cell cycle progression by activating CDK2 protein expression. Besides, MEF2A was found to be an important transcription factor that bound to the myozenin 2 (MyoZ2) proximal promoter and performed upstream of MyoZ2 during myoblast differentiation. This study provides the first experimental evidence that MEF2A is a positive regulator in skeletal muscle myoblast proliferation and suggests that MEF2A regulates myoblast differentiation via regulating MyoZ2.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据