期刊
PLOS ONE
卷 13, 期 1, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0189616
关键词
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资金
- Department of Energy Office of Science, Office of Biological AMP
- Environmental Research Genomic Science Program [DE-SC0004486, DE-SC0010558]
- U.S. Department of Energy (DOE) [DE-SC0010558] Funding Source: U.S. Department of Energy (DOE)
Combining high throughput sequencing with stable isotope probing (HTS-SIP) is a powerful method for mapping in situ metabolic processes to thousands of microbial taxa. However, accurately mapping metabolic processes to taxa is complex and challenging. Multiple HTS-SIP data analysis methods have been developed, including high-resolution stable isotope probing (HR-SIP), multi-window high-resolution stable isotope probing (MW-HR-SIP), quantitative stable isotope probing (qSIP), and ABD. Currently, there is no publicly available software designed specifically for analyzing HTS-SIP data. To address this shortfall, we have developed the HTSSIP R package, an open-source, cross-platform toolset for conducting HTS-SIP analyses in a straightforward and easily reproducible manner. The HTSSIP package, along with full documentation and examples, is available from CRAN at https://cran.rproject.org/web/packages/HTSSIP/index. html and Github athttps://github.com/buckleylab/HTSSIP.
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