期刊
PLOS ONE
卷 13, 期 3, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0193834
关键词
-
资金
- Ministry of Health, Welfare and Sports
- Ministry of Economic Affairs of the Netherlands
- Lung Foundation Netherlands [3.2.11.022]
A subset of the study population from a cross sectional study of carriership of ESBU pAmpC producing E. coli (ESBL E) in the general population was followed up by five successive samples over an approximate half year period, leading to six samples in 333 persons. Fecal samples were cultured and analyzed for the presence of E. coli types as characterized by MLST, and ESBUpAmpC genes were analysed by PCR and sequencing. The study included 255 persons who had a negative first sample, to allow observations of acquiring carriership of ESBL E. Any individual record thus consisted of a series of snapshots of episodes of presence and absence of ESBL E carriage. A survival model was built to estimate times to acquire or lose carriership, allowing for any combination of ESBU pAmpC gene and E. coli MLST type. In carriers, the mean time to lose carriership was 1.1 (95% range 0.8-1.6) years. The estimated mean time to acquire carriership was 3.0 (95% range 1.6-6.3) years. Analysis of these times by ESBUpAmpC gene found substantial variation among resistance genes both in persistence of carriership and in rates of acquiring carriership:, bla(CTX-M-1), bla(CTX-M-14), bla(CTX-M-15) bla(CTX-M-27) and bla(SHV-12) were easily acquired, but bla(CTX-M-1) and bla(SHV-12) were also easily lost, while bla(CTX-M-15), bla(CTX-M-27) and bla(CMY-2) were more likely to persist. When in addition bacterial host types were included, some combinations appeared more persistent than others (bla(CTX-M-1) in ST10 and ST58; bla(CMY-2) , bla(CMY-2), and bla(SHY-12) in ST69), or were acquired with higher frequency (bla(CTX-M-1), bla(CTX-M-14), bla(CTX-M-15) bla(CTX-M-27) and bla(SHV-12) in ST131; blasHv_12 in ST69). The relatively short duration of carriership means that when an intervention drastically reduces the exposure of humans to ESBL-E, the prevalence will be halved in 0.66 years. The observed differences between carriage rates of ESBUpAmpC genes and E. coli strains need further investigation.
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