期刊
BIORESEARCH OPEN ACCESS
卷 2, 期 2, 页码 118-127出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/biores.2012.0300
关键词
atherosclerosis; DNA methylation; hyperhomocysteinemia; monocyte chemoattractant protein-1
资金
- Specialized Research Fund for the National Natural Science Foundation of China [30960124, 81160044]
- New Century Excellent Talents in University [NCET-10-0916]
Hyperhomocysteinemia is considered to be a significant risk factor in atherosclerosis and plays an important role in it. The purpose of this study was to determine the molecular mechanism of blood monocyte chemoattractant protein-1 (MCP-1) promoter DNA hypomethylation in the formation of atherosclerosis induced by hyperhomocysteinemia, and to explore the effect of nuclear factor-kappa B (NF-kappa B)/DNA methyltransferase 1 (DNMT1) in this mechanism. The atherosclerotic effect of MCP-1 in apolipoprotein E-deficient (ApoE(-/-)) and wild-type C57BL/6J mice was evaluated using atherosclerotic lesion area; serum NF-kappa B, MCP-1, and DNMT1 levels; and MCP-1 promoter DNA methylation expression. In vitro, the mechanism responsible for the effect of NF-kappa B/DNMT1 on foam cells was investigated by measuring NF-kappa B and DNMT1 levels to determine whether NF kappa B/DNMT1 had an effect on gene expression. Compared with the control group, atherosclerotic lesions in ApoE(-/-) mice fed a high methionine diet significantly increased, as did the expression of MCP-1. In vitro study showed that pyrrolidine dithiocarbamate treatment down-regulated levels of NF-kappa B and raised DNMT1 concentrations, confirming the effect of NF-kappa B/DNMT1 in the MCP-1 promoter DNA methylation process. In conclusion, our results suggest that through NF-kappa B/DNMT1, MCP-1 promoter DNA hypomethylation may play a key role in formation of atherosclerosis under hyperhomocysteinemia.
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