Enhancement of cellulase activity by a new strain of Thermoascus aurantiacus: Optimisation by statistical design response surface methodology

Thermophilic fungi Thermoascus aurantiacus RBB1 isolated from composting soil was optimised for production of cellulolytic enzymes (endoglucanase, filter paper cellulase by SSF). Initial experiments showed that culture medium containing wheat bran as carbon source, prepared in a synthetic basal medium, supported maximum enzyme production at 50 C-circle. Further optimisation of enzyme production was carried out using Box-Behnken design of experiments, to study the influence of process variables such as ammonium sulphate, moisture ratio, initial pH and inoculum level on enzyme production. The response surface plots revealed the conditions for obtaining optimal enzyme levels. The models computed for R-2 ( adj R-2) values were 92.6% (84.0%), 95.8% (91.0%), indicating they are appropriate and can be useful to predict the effect of ammonium sulphate concentration, moisture ratio, initial pH and inoculum level on production of endo-beta-1,4-glucanase and filter paper cellulase. Under optimised conditions 420.8 and 22.73 units/g substrate of endo-beta-1,4-glucanase and filter paper cellulase were produced, respectively. (C) 2013 Elsevier Ltd. All rights reserved.