期刊
PLANT SCIENCE
卷 272, 期 -, 页码 107-116出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2018.04.010
关键词
Chlamydomonas; ER; Gene expression; Heat-shock protein; HSP90; Promoter analysis
资金
- Thailand Research Fund [RSA5680032, MRG6080235]
- Mahidol University
- Kasetsart University Research and Development Institute [vt-d 49.57]
- Royal Society-OHEC Newton Mobility Grant [NI160206]
- Science Achievement Scholarship of Thailand [SAST]
Molecular chaperones or heat shock proteins are a large protein family with important functions in every cellular organism. Among all types of the heat shock proteins, information on the ER-localized HSP90 protein (HSP90B) and its encoding gene is relatively scarce in the literature, especially in photosynthetic organisms. In this study, expression profiles as well as promoter sequence of the HSP90B gene were investigated in the model green alga Chlamydomonas reinhardtii. We have found that HSP90B is strongly induced by heat and ER stresses, while other short-term exposure to abiotic stresses, such as salinity, dark-to-light transition or light stress does not appear to affect the expression. Promoter truncation analysis as well as chromatin immunoprecipitation using the antibodies recognizing histone H3 and acetylated histone H3, revealed a putative core constitutive promoter sequence between -1 to - 253 bp from the transcription start site. Our results also suggested that the nucleotides upstream of the core promoter may contain repressive elements such as putative repressor binding site(s).
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据