Review: Plant-pathogen interactions through the plasmodesma prism

Plasmodesmata (PD) allow membrane and cytoplasmic continuity between plant cells, and they are essential for intercellular communication and signaling in addition to metabolite partitioning. Plant pathogens have evolved a variety of mechanisms to subvert PD to facilitate their infection of plant hosts. PD are implicated not only in local spread around infection sites but also in the systemic spread of pathogens and pathogen-derived molecules. In turn, plants have developed strategies to limit pathogen spread via PD, and there is increasing evidence that PD may also be active players in plant defense responses. The last few years have seen important advances in understanding the roles of PD in plant-pathogen infection. Nonetheless, several critical areas remain to be addressed. Here we highlight some of these, focusing on the need to consider the effects of pathogen-PD interaction on the trafficking of endogenous molecules, and the involvement of chloroplasts in regulating PD during pathogen defense. By their very nature, PD are recalcitrant to most currently used investigative techniques, therefore answering these questions will require creative imaging and novel quantification approaches.