期刊
PLANT PHYSIOLOGY AND BIOCHEMISTRY
卷 131, 期 -, 页码 22-30出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2018.03.027
关键词
Genome editing; Engineered or synthetic nucleases; Zinc Finger Nucleases (ZFNs); Transcription Activator Effector-Like Nucleases (TALENs); Clustered Regularly Interspaced Short; Palindromic Repeats (CRISPR); Endonucleases
资金
- Science and Engineering Research Board (SERB)
- DST, Government of India, New Delhi (Early Career Research Grant) [YSS/2015/000616]
- National Research Foundation of Korea, Republic of Korea [2017R1A4A1015515]
Genome editing (GE) tools ensure targeted mutagenesis and sequence -specific modification in plants using a wide resource of customized endonucleases; namely, zinc-finger nucleases (ZFNs), and transcription activator like effector nucleases (TALENs), and the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated protein) system. Among these, in recent times CRISPR/Cas9 has been widely used in functional genomics and plant genetic modification. A significant concern in the application of GE tools is the occurrence of 'off-target' activity and induced mutations, which may impede functional analysis and gene activity studies. Moreover, the 'off-target' activity results in either not reported or unknown, difficult to detect, produce non-quantifiable cellular signaling and physiological effects. In the past few years, several experimental methods have been developed to identify undesired mutations and to curtail 'off-target' cleavage. Improvement in target specificity and minimizing 'off-target' activity will offer better applications of GE technology in plant biology and crop improvement.
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