期刊
PLANT PHYSIOLOGY AND BIOCHEMISTRY
卷 127, 期 -, 页码 211-222出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2018.03.028
关键词
Cucumber; Phosphate; Phosphate transporter; Protein; Starch; Sugar
资金
- Khalifa Center for Biotechnology and Genetic Engineering-UAEU Grant [31R081]
- UAEU Program for Advanced Research Grant [31S255]
Although abundantly present in soils, inorganic phosphate (Pi) acquisition by plants is highly dependent on the transmembrane phosphate transporter (PT) gene family. Cucumber (Cucumis sativus) requires a large amount of phosphorus (P). The purpose of this study was to isolate the CsPT2-1 from cucumber roots, and to determine the influence of Pi nutrition on cucumber growth, metabolism and transcript levels of CsPT2-1 in tissues. Full length CsPT2-1 was cloned and phylogenetically identified. In two greenhouse experiments, P-deficient seedlings provided with low or high P concentrations were sampled at 10 and 21 days post treatment, respectively. Addition of P dramatically reduced growth of roots but not shoots. Supplying plants with high P resulted in increased total protein in leaves. Acid phosphatase activity increased significantly in leaves at any rate higher than 4 mM P. Increasing P concentration had a notable decrease in glucose concentrations in leaves of plants supplied with > 0.5 mM P. In roots, glucose and starch concentrations increased with increasing P supply. Steady state transcript levels of CsPT2-1 were high in P-deprived roots, but declined when plants were provided > 10 mM P. To our knowledge, this is the first report focusing on a PT and its expression levels in cucumber.
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