期刊
PLANT PHYSIOLOGY
卷 177, 期 2, 页码 476-489出版社
AMER SOC PLANT BIOLOGISTS
DOI: 10.1104/pp.18.00293
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资金
- Internal Grant Agency of Palacky University [IGA_PrF_2018_023]
- Ministry of Education, Youth, and Sports of the Czech Republic (National Program for Sustainability I) [LO1204]
- Czech Science Foundation [GA17-06613S]
- Swedish Governmental Agency for Innovation Systems (Vinnova)
- Swedish Research Council
Phytohormones are physiologically important small molecules that play essential roles in intricate signaling networks that regulate diverse processes in plants. We present a method for the simultaneous targeted profiling of 101 phytohormone-related analytes from minute amounts of fresh plant material (less than 20 mg). Rapid and nonselective extraction, fast one-step sample purification, and extremely sensitive ultra-high-performance liquid chromatography-tandem mass spectrometry enable concurrent quantification of the main phytohormone classes: cytokinins, auxins, brassinosteroids, gibberellins, jasmonates, salicylates, and abscisates. We validated this hormonomic approach in salt-stressed and control Arabidopsis (Arabidopsis thaliana) seedlings, quantifying a total of 43 endogenous compounds in both root and shoot samples. Subsequent multivariate statistical data processing and cross-validation with transcriptomic data highlighted the main hormone metabolites involved in plant adaptation to salt stress.
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