期刊
PLANT JOURNAL
卷 94, 期 6, 页码 1010-1022出版社
WILEY
DOI: 10.1111/tpj.13914
关键词
alternative splicing; thermo-sensitivity; cyclin-dependent kinases; Arabidopsis
资金
- BBSRC [BB/M009459/1]
- VIPS Program of the Austrian Federal Ministry of Science and Research
- City of Vienna
- Austrian Science Fund (FWF) [DK W1207, SFB RNAreg F43-P10]
- BBSRC [BB/M009459/1] Funding Source: UKRI
- Austrian Science Fund (FWF) [W1207] Funding Source: Austrian Science Fund (FWF)
The ability to adapt growth and development to temperature variations is crucial to generate plant varieties resilient to predicted temperature changes. However, the mechanisms underlying plant response to progressive increases in temperature have just started to be elucidated. Here, we report that the cyclin-dependent kinase G1 (CDKG1) is a central element in a thermo-sensitive mRNA splicing cascade that transduces changes in ambient temperature into differential expression of the fundamental spliceosome component, ATU2AF65A. CDKG1 is alternatively spliced in a temperature-dependent manner. We found that this process is partly dependent on both the cyclin-dependent kinase G2 (CDKG2) and the interacting co-factor CYCLIN L1 (CYCL1), resulting in two distinct messenger RNAs. The relative abundance of both CDKG1 transcripts correlates with ambient temperature and possibly with different expression levels of the associated protein isoforms. Both CDKG1 alternative transcripts are necessary to fully complement the expression of ATU2AF65A across the temperature range. Our data support a previously unidentified temperature-dependent mechanism based on the alternative splicing (AS) of CDKG1 and regulated by CDKG2 and CYCL1. We propose that changes in ambient temperature affect the relative abundance of CDKG1 transcripts, and this in turn translates into differential CDKG1 protein expression coordinating the AS of ATU2AF65A.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据