期刊
PLANT CELL
卷 30, 期 6, 页码 1243-1257出版社
AMER SOC PLANT BIOLOGISTS
DOI: 10.1105/tpc.18.00011
关键词
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资金
- Institut National de La Recherche Agronomique
- National Science Foundation [IOS 1339362]
- Agence Nationale de la Recherche [IMANA ANR-14-CE19-0008]
- Czech Science Foundation [GA17-06613S]
- Ministry of Education, Youth, and Sports of the Czech Republic [LO1204]
Plants face temporal and spatial variation in nitrogen (N) availability. This includes heterogeneity in soil nitrate (NO3-) content. To overcome these constraints, plants modify their gene expression and physiological processes to optimize N acquisition. This plasticity relies on a complex long-distance root-shoot-root signaling network that remains poorly understood. We previously showed that cytokinin (CK) biosynthesis is required to trigger systemic N signaling. Here, we performed split-root experiments and used a combination of CK-related mutant analyses, hormone profiling, transcriptomic analysis, NO3- uptake assays, and root growth measurements to gain insight into systemic N signaling in Arabidopsis thaliana. By comparing wild-type plants and mutants affected in CK biosynthesis and ABCG14-dependent root-to-shoot translocation of CK, we revealed an important role for active trans-zeatin (tZ) in systemic N signaling. Both rapid sentinel gene regulation and long-term functional acclimation to heterogeneous NO3- supply, including NO3- transport and root growth regulation, are likely mediated by the integration of tZ content in shoots. Furthermore, shoot transcriptome profiling revealed that glutamate/glutamine metabolism is likely a target of tZ root-to-shoot translocation, prompting an interesting hypothesis regarding shoot-to-root communication. Finally, this study highlights tZ-independent pathways regulating gene expression in shoots as well as NO3-uptake activity in response to total N deprivation.
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