期刊
PLANT BIOTECHNOLOGY JOURNAL
卷 17, 期 1, 页码 132-140出版社
WILEY
DOI: 10.1111/pbi.12952
关键词
CRISPR/Cas9; DELLA; gibberellic acid; dwarf; slender; PROCERA
资金
- Bill and Melinda Gates Foundation (Grand Challenges Exploration) [OPP1060026]
- Biotechnology and Biological Sciences Research Council (BBSRC) [BB/L009293/1]
- US Israel Binational Agriculture Research and Development Fund [US-4813-15C]
- Gatsby Charitable Foundation
- BBSRC [BBS/E/J/000PR9795, BB/L009293/1] Funding Source: UKRI
The tomato PROCERA gene encodes a DELLA protein, and loss-of-function mutations derepress growth. We used CRISPR/Cas9 and a single guide RNAs (sgRNA) to target mutations to the PROCERA DELLA domain, and recovered several loss-of-function mutations and a dominant dwarf mutation that carries a deletion of one amino acid in the DELLA domain. This is the first report of a dominant dwarf PROCERA allele. This allele retains partial responsiveness to exogenously applied gibberellin. Heterozygotes show an intermediate phenotype at the seedling stage, but adult heterozygotes are as dwarfed as homozygotes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据