期刊
PLANT AND CELL PHYSIOLOGY
卷 59, 期 5, 页码 1027-1042出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcy044
关键词
Ethylene response factor; Hub genes; Leaf color; RNA-seq; SPL; Weighted gene co-expression network analysis
资金
- National Natural Science Foundation of China [31501723]
- Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges Under Beijing Municipality [IDHT20180509]
- Importation and Development of High-Caliber Talents Project of Beijing Municipal Institutions
- Beijing Technology Innovation Service Capacity Construction-Research Plan [KM201610020003]
- Scientific Research Improvement Project of BUA [GZL2017005]
Anthocyanins are plant pigments that contribute to the color of leaves, flowers and fruits, and that are beneficial to human health in the form of dietary antioxidants. The study of a transformable crabapple cultivar, 'India magic, which has red buds and green mature leaves, using mRNA profiling of four leaf developmental stages, allowed us to characterize molecular mechanisms regulating red color formation in early leaf development and the subsequent rapid down-regulation of anthocyanin biosynthesis. This analysis of differential gene expression during leaf development revealed that ethylene signaling-responsive genes are up-regulated during leaf pigmentation. Genes in the ethylene response factor (ERF), SPL, NAC, WRKY and MADS-box transcription factor (TF) families were identified in two weighted gene co-expression network analysis (WGCNA) modules as having a close relationship to anthocyanin accumulation. Analyses of network hub genes indicated that SPL TFs are located in central positions within anthocyanin-related modules. Furthermore, cis-motif and yeast one-hybrid assays suggested that several anthocyanin biosynthetic or regulatory genes are potential targets of SPL8 and SPL13B. Transient silencing of these two genes confirmed that they play a role in co-ordinating anthocyanin biosynthesis and crabapple leaf development. We present a high-resolution method for identifying regulatory modules associated with leaf pigmentation, which provides a platform for functional genomic studies of anthocyanin biosynthesis.
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