期刊
PLACENTA
卷 61, 期 -, 页码 103-105出版社
W B SAUNDERS CO LTD
DOI: 10.1016/j.placenta.2017.12.001
关键词
Placenta; Trophoblast; Sex determination; Genomic DNA; PCR
资金
- ANSES (PhthalatPreG project)
It is now demonstrated that the sex-specific maternal-placental-fetal interaction plays an important role in placental functions and pathologies. Determination of fetal-sex may therefore be an important consideration in studies using placenta samples. In this present study, we describe a simple, fast, and cheap protocol, which allows the fetal-sex determination of placental tissues from various starting materials (villi or formalin-fixed, paraffin-embedded (FFPE) tissues, isolated cytotrophoblasts or cellular debris from whole cell lysates, and cDNA) by a single duplex PCR reaction followed by agarose gel electrophoresis. (c) 2017 Elsevier Ltd. All rights reserved.
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