期刊
PHOTOSYNTHESIS RESEARCH
卷 137, 期 2, 页码 295-305出版社
SPRINGER
DOI: 10.1007/s11120-018-0504-1
关键词
Chlorophototrophy; Reaction centers; Chloracidobacterium thermophilum; N-15-MAS NMR; Photo-CIDNP; Zn-BChl a '
资金
- Deutsche Forschungsgemeinschaft DFG [MA4972/2-1]
- Photosynthetic Systems Program, Division of Chemical Sciences, Geosciences, and Biosciences (CSGB), Office of Basic Energy Sciences of the U.S. Department of Energy [DE-FG02-94ER20137, DE-SC0010575]
- ERC Starting Grant 'PhotoMutant' [678169]
- ERC [GA 615217]
- ISF [GA 558/14]
- U.S. Department of Energy (DOE) [DE-SC0010575] Funding Source: U.S. Department of Energy (DOE)
Photochemically induced dynamic nuclear polarization (photo-CIDNP) has been observed in the homodimeric, type-1 photochemical reaction centers (RCs) of the acidobacterium, Chloracidobacterium (Cab.) thermophilum, by N-15 magic-angle spinning (MAS) solid-state NMR under continuous white-light illumination. Three light-induced emissive (negative) signals are detected. In the RCs of Cab. thermophilum, three types of (bacterio)chlorophylls have previously been identified: bacteriochlorophyll a (BChl a), chlorophyll a (Chl a), and Zn-bacteriochlorophyll a' (Zn-BChl a') (Tsukatani et al. in J Biol Chem 287:5720-5732, 2012). Based upon experimental and quantum chemical N-15 NMR data, we assign the observed signals to a Chl a cofactor. We exclude Zn-BChl because of its measured spectroscopic properties. We conclude that Chl a is the primary electron acceptor, which implies that the primary donor is most likely Zn-BChl a'. Chl a and 8(1)-OH Chl a have been shown to be the primary electron acceptors in green sulfur bacteria and heliobacteria, respectively, and thus a Chl a molecule serves this role in all known homodimeric type-1 RCs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据